Chemistry Journal of Moldova

CONSIDERATIONS ON LIQUID-CHROMATOGRAPHIC SEPARATION FOR AN EQUIMOLAR MIXTURE OF 2,4-DINITROPHENYLHYDRAZONES OF ACETALDEHYDE AND DIACETYL

Author(s):

Gheorghe Zgherea


Field: Analytical chemistry
Type: Research paper
Issue: 2008 Volume 3, no.1
Pages: 48-51
Keywords:

acetaldehyde, diacetyl, reverses phase, polarity, percent of water, inversion of position.


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Abstract (JPEG)

DOI:
dx.doi.org/10.19261/cjm.2008.03(1).03

Graphical Abstract:
An equimolar mixture of 2.4-dinitrophenylhidrazones (2.4DNPH-ones) providing by acetaldehyde and diacetyl must be analyzed by liquid-chromatographic separation, using the mechanism of repartition with reverse phase; that full papers is for identification the optimal analytical conditions. As mobile phase are utilized various binary mixtures eluent, containing water and methanol, with 0-45% water. By the experimental studies were identified four domains of behavior and two optimal binary mixtures, with 25% and 45% water, thus this is a study on the behavior of binary mixtures mobile phases. The peaks are characterized by values of retention times and by position. The separation processes were appreciated by difference between the retention times of peaks; if the percent of water increase, the values of retention times is higher. When the percent of water is 45%, the difference between the retention times is maxim, associated with a change of peaks position.

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